Should FMN switch dot plots be biased towards bound state? This paper suggests the answer may be yes.
And further it suggests as I have maintained—
“The average
observed melting point ™ calculated from the two-state
folding transition of each measurable nucleotide (see
‘Materials and Methods’ section) slightly increased upon
ligand binding [average Tm±standard deviation (SD)]:
64.9±4.6 C (unbound) and 69.3±4.3 C (FMN bound)”—
melting point should go up when bound, or at least not go down.