Gross Berex Ratio = GBR = (Non_AU)/AU = (ideal)55/45 & both numerator & denominator are fib or sums of fib numbers.
1,1,2,3,5,8,13,21,44,65…Total Nt’s = sum of four fib numbers,i.e.,
100=(21+21+13+(44+1)) each nt-group A,U,G,C is a fib or sum of fib number.
(Maybe limit nt-group A,U,G,C to sum of two fib numbers.)
FE = Free energy = 24 * GBR (actual)
FE * 1.6 + 24 < Melt Temp < 107
0.1 < FE/Melt temp < 1 (small RNA < 200nt’s) (Maybe omit this)
(could be as AND/OR/WHILE relation to Melt temp above).
Clean Dot plot = more than 75% of upper right triangle dot plot probability is found in expected base pairs.
And/OR
Lowest actual expected base pair probability >= any miss paired probability.
Eliminate rules from bottom up if needed to optimize.