From DPAT3 penalize designs that have any of the following list of stacks in R96 Same State NG 3 since they are only predicted by Vienna 2 to exist in the MFE structure of poor performing designs only ( designs scoring bellow 80 only). Here is an explanation from DPAT3’s documentation of the way the stacks are written)
’ If you wanted to look at a stack in the 2nd State with 3 pairs in it and the nucleotide pairs are 22 paired with 50, 23 paired with 49, and 24 paired with 48 then it would be written, “2ndState-22:50,23:49,24:48" ’
And here are the 1949 unique stacks predicted only in designs scoring under 80 in R96 Same State NG 3
https://dl.dropboxusercontent.com/u/87351147/R96SameStateNG3_SNuPP_Pickstacks_range0-80.TXT .