What is the concentration of the OpenTB design sequences for input to NUPACK?

What are the concentrations of the design sequences in the OpenTB labs? I am coding up concentrations in NUPACK and the example show a concentration used for the main sequence.


Thermodynamic parameters, like the Turner 1999 & 2004 ones, generally assume a concentration of 1M (1 molar) and that’s how the puzzles are modeled in the game, which I remind you, is MFE-oriented.

When working with partition functions though, things are a bit different and if you haven’t already, I’d suggest reading my old blog post about the [A]/[B] lab.

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