Please review the proposed puzzles for the initial OpenCRISPR round

113

You also need to remove the cookies and the chache from what I understand.

115

For me, after deleting the cookies (only read this after I deleted them), it appears in ON and doesn’t in OFF, as it should. However this 40k loop is a pretty weird quirk in the engine…

117

I cleared the chache as well as the history, called up the puzzle, reset the puzzle, then pasted the solution and that did it.  

Thanks so much.

118

TEP 1a - Same State
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAGAGAUACCAGAUCAAUCUGGUAUAAGAAUUGACAUGAGGAUCACCCAUGUGAAUAAAGACAGAAUAAGUGAUCCCUUGGCAGCUCUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

119

TEP 1a - Exclusion
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACAGAUACCAGACAUUCUGGUAUAGAUCUCAGAUGUCAUUGACAUCUGAGAUCUACAUGAGGAUCACCCAUGUCCCUUGGCAGCUGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

120

TEP 1b - Same State
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAGACCCUUGGCAGCAUGAAACAUGAGGAUCACCCAUGUAGAUGAGACAUUUGUCUCAUCUUCGUUUUGAACCUCAUGAUACCAGUCUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

121

TEP 1b - Exclusion
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACACCCUUGGCAGCACAUUAAAGAUCUCUCGAGUCAUUUGACUCGAGAGAUCAAAUUACAUGAGGAUCACCCAUGUGAUACCAGUGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

122

TEP 2 - Same State
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACAGAACAUGAGGAUCACCCAUGUAAAAUGAUACCAGCAGAUUUCUGCCCUUGGCAGCAUGGGUAGAGCGAUUUCGCUCAAAACUGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

123

TEP 2 - Exclusion
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACAGUAUAUGAGUGAACACAUCGGAAGGUGAUACCAGCUGAUUUCAGCCCUUGGCAGCACAUGAGGAUCACCCAUGUAAUAUACUGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Ugly solution works in all 3 models.

124

TEP 3 - Same State
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACAGAAAAUGAUACCAGAGCAUUUGCUCCCUUGGCAGCAUGUGUAUAAUUAUACACAUGAGGAUCACCCAUGUAAGAAAUAAUCUGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

125

TEP 3 - Exclusion
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACGGAUGUGGAUACCAGCUCAUUUGAGCCCUUGGCAGCUACAUGAGGAUCACCCAUGUAAGAUCUCUCAUUUGAGAGAUCAAACCGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

126

TEP 4 - Same State
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAGAGCAAUAAAUAAUAAACAUGAGGAUCACCCAUGUGAUAAAAUCACAUGAUACCAGCUCAUUUGAGCCCUUGGCAGCAUAAGCUCUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

127

TEP 4 - Exclusion
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAGACAUAAUUGCUCGCAUUUGUGAGCACACACAUGAGGAUCACCCAUGUGAUACCAGCUCAUUUGAGCCCUUGGCAGCACAUUGUCUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

128

Tetracycline - Same State
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACUAAAACAUACCUUAUGUAAAUAAAACAUGAGGAUCACCCAUGUAAAUAAAUAUAUAGGGAGAGGUGAAGAAUACGACCACCUAGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
This solution was not a mod but turned out to be very similar to Eli’s, both work in all 3 models.

129

Tetracycline - Exclusion
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACUAAAACAUACCACAUGAGGAUCACCCAUGUAAAAGAGCUCAUUUGAGCUCAAAGUGUGGAGAGGUGAAGAAUACGACCACCUAGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

130

Tetracycline inv - Same State
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAGGAGAGGUGAAGAAUACGACCACCUAGGAAUCUCAUGUAAAACAUGAGGAUCACCCAUGUAAAUUGAAUGAUCCUAAAACAUACCUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

131

Tetracycline inv - Exclusion
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAGGAGAGGUGAAGAAUACGACCACCUAGUACAUGAGGAUCACCCAUGUAAAACAUGUAAAUUAAAUAUGAUGUACUAAAACAUACCUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

132

Control ON
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAUAAUAAUAGCUAUAUAGAAAUAUAUAGGAUAUAACAUGAGGAUCACCCAUGUUAUAUCGAAUAUAGAAAUAUAUUCCUAUUAUUAUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

Control OFF
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACUCUCUGACCAAUCUCGAAAGAGAUUGUCAUGUACAUGAGGGAAACCCAUGUACAUGAGGAUCACCCAUGUGAUCCGUCAGAGAGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

Control ON (freestyle)
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUAUAACUAAAGCUCAGAAGAAAUUCUGAGGUUAGAACAUGAGGAUCACCCAUGUUCUAACGACUAGAGAAAUCUAGUCCUUUAGUUAUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

Control OFF (freestyle)
GACGCAUAAAGAUGAGACGCGUUUGAGAGCUACUGUCUGACCAAUCUCGAAAGAGAUUGUCAUGUACAUGAAAGAAACCCAUGUACAUGAGGAUCACCCAUGUGAUCCGUCAGACAGUAGCAAGUUCAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUU
Solution works in all 3 models.

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133

Also for those who are not doing puzzles at the moment and may not have noticed, there is a CRISPR training series up under puzzles. The aim is teaching some of the skills for doing good for the coming CRISPR lab. With focus on using what we have already learned from past labs + transfering some of our past winning designs to the CRISPR lab as for making potential winners much faster.

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134

First of all, I want to thank everyone who has helped us test these puzzles.  The feedback resulted in numerous very significant improvements and fixes.  Special kudos are due to Andrew, who submitted solutions satisfying all three folding engines, for all the final puzzle versions!

Barring any last minute surprises, the puzzles are now finalized.  If you are so inclined, you can start working on them now; you just have to keep a copy of your solutions and submit them when the round officially starts.  (When?  Soon, but exact date still TBD.)  But this pause brings up another thought.

Once a lab opens, we are generally too busy working on generating lots of solutions to take the time to discuss our ideas for how to best solve them.  I think we might get a jump on new puzzles if we shared ideas on how we think we can best apply lessons from the past.  Eli has jump-started this with his player puzzle tutorial series.  Are there other thoughts?  @Andrew, you’ve obviously gotten very good at generating high quality solutions.  Can you share anything about your process?

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